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[Huggybaby]

Should I install 20100109 update over 2010 ISO?

Nope, that's not how it works.
I'm sure this is confusing to those who haven't been aware of Manalink's advancements over the years. Here's an approximate TimeLine:

1997) Original Microprose MtG is released
1998) Microprose MtG: Duels of the Planeswalkers is released
2001) Mok releases his Manalink 1.3.2 Unofficial Update
2006) Mok releases his final Manalink 2.0 Mod, and then promptly disappears
2007) Community Updates to Manalink 2.0 begin
2009) New graphics and GameModes added to Manalink
2010) 2000 card limit is reached and old cards start being removed
2010) The CCGHQ Custom ISO is released
2011) Mok briefly reappears and helps Devs to break the 2000 card limit
2012) Manalink 3.0 is released (now with a 16384 card limit)

Manalink has seen many changes and additions over the years, so properly updating a basic Microprose install can be a real difficult and convoluted pain... hence the complete updated versions. Although they all use MtG DotP + Manalink 1.3.2 as a base, once updated they're quite incompatible with each other... especially the 2010 Custom ISO. It has a few custom updates of it's very own, and you should never use any other updates on that version.

There are several "complete" versions available:

Manalink 1.3.2 - The original Microprose MtG: Duels of the Planeswalkers with all 649 cards enabled, direct peer-to-peer network connections, and several fixes to Shandalar that make it playable on 'modern systems' (i.e. anything faster than 300Mhz). This version has been compatible with every edition of Windoze I've ever owned (95b, 98SE, 2000, XPsp3, 7x64) and I still play it occasionally.
Manalink 1.3.2 update is dated August 12, 2001.

CCGHQ 2010 Custom ISO - No new Game Modes or ArtWork (other than CardArt), but this version is quick, responsive, and stable as hell. It looks, acts, plays, and installs just like the original game, but contains more than twice the number of cards! I believe the 2010 ISO contains all of the updates that were written in ASM, before C programming was enabled for new cards (drastically simplifying and speeding up the process). Still my favorite version and probably the one I play the most.
The last update for the CCGHQ Custom ISO was July 21, 2011.

Manalink 2.0 - Since it was in constant development by several people over many years, there are simply oodles and oodles of updates for this version. It introduced all of the current extra Game Modes (Momir, Challenge, Draft, etc.) as well as all of the improved graphics and CardArt updates. It also used separate executables to work around the 2000 card limit (Magic.exe, MagicLimited.exe, MagicCustom.exe, and MagicGY.exe), with each executable requiring a separate CardArt Folder.
The last update for Manalink 2.0 was December 1, 2011.

Manalink 3.0 - The current version was officially released on January 28, 2012 and is developed by Gargaroz. It uses a completely different CARDS.DAT format than previous versions to accommodate the expanded card list and also contains an updated DeckBuilder, new CardArt file naming format, plus a few other graphic updates. The version found in my MtG Folder has Shandalar completely removed.
The March 22, 2013 update contains 7437 coded cards and 9515 CardArt images.

Shandalar 2012 - Shandalar.exe (the "adventure" part of the game) was updated in 2001 as part of Mok's Manalink 1.3.2, and didn't receive another update until December 2011. No new cards were added, but it now uses the updated DeckBuilder, CardArt, and DuelArt from Manalink 3.0 and has pre-juiced SaveFiles... other than that, it's the same ol' Shandalar. Great as ever. ^_^

These versions should all be considered separate entities, and you should always use the appropriate Base Install when updating.

Oh, about Manalink 2.x 20100109. That's approximately the last version of Manalink 2.0 before the inevitable removal of older cards to make room for new ones (because of the 2000 card limit). It comes complete with Game Modes and updated ArtWork, etc., and retains backwards-compatibility with all of the original PlayDecks (which is no longer an issue with Manalink 3.0). If desired, it can be used as a Base Install for further updates of Manalink 2.0 (never go backwards).

Newest downloads can be found at the links in my signature. I'm currently planning on rebuilding the 2.x 20100109 version (because the PlayDeck Analyser has exposed much incorrectness) and Shandalar is about to get a Major professional-style upgrade courtesy of hip63 (who built the 2010 Custom ISO installer). I've just seen the Beta, and brother... spectacular doesn't even begin to describe it!

Personal Note: Nobody is gonna read that wall of text. ^_^

This is an excellent post, thank you. My reply is kind of necro but the thread deserves it because the project is so noteworthy and I love it for many reasons. The 2010 ISO has helped uncounted numbers of people and has been mirrored all over the place. IMO it's the standard install of MTG: DOTP internet-wide.

Unfortunately, so many dead links in the first post...

[Aswan jaguar]

I have uploaded the missing Microprose MTG Mok's Manalink 2.0 Version.part1.rar here:
https://www.mediafire.com/file/9u1b07ux ... 1.rar/file
I also have the Version.part2.rar but I did a check at least for files and sizes and doesn't seem that 2nd part has anything that the 1st part hasn't. Unless it has a couple of files with the exact same size but different content? Was the 2nd part supposed to be installed over the first?
Also checked a bit and runs fine in windows 10 pro 64bit.

[Huggybaby]

Thanks Aswan!